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Title: | Preparation of Eel (Monopterus Albus) Protein Hydrolysate Using Alcalase® And Its Physicochemical, Antioxidant And Anticancer Properties |
Authors: | Nura Ruhaya Binti Abdul Halim |
Keywords: | Nura Ruhaya Abdul Halim Eel protein hydrolysate Physicochemical |
Issue Date: | Oct-2017 |
Publisher: | Universiti Malaysia Terengganu |
Abstract: | The study aims to determine the optimum condition of eel protein hydrolysate (EPH) produced using Alcalase® and its physicochemical, antioxidant and anticancer properties. The hydrolysis condition was optimized using Response Surface Methodology (RSM) by applying four factors (time, temperature, pH, Alcalase® concentration), 3-levels Central Composite Design (CCD) with six central points. The chemical and amino acid compositions, structural properties, solubility, emulsifying and foaming properties, water holding and fat binding capacity of EPH were determined. The EPH was separated through ultrafiltration membranes (10 kDa, 5 kDa and 3 kDa). The ferric thiocyanate, thiobarbituric reactive substance, reducing power, ferrous ion chelating activity and 1,2-diphenylpicrylhydrazyl radical scavenging activity of fractionated EPH were also determined. The anticancer activity was determined using MCF-7 cell lines. In order to obtain the optimum yield, degree of hydrolysis (DH) and DPPH activity, the optimum hydrolysis condition suggested was 84.02 min, 50.18°C, pH 7.89 and 2.26% Alcalase® concentration |
URI: | http://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/14255 |
Appears in Collections: | Pusat Pengajian Sains dan Teknologi Makanan |
Files in This Item:
File | Description | Size | Format | |
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Full Text SH 351 .E4 N8 2017.pdf Restricted Access | 1.1 MB | Adobe PDF | View/Open Request a copy | |
Abstract SH 351 .E4 N8 2017.pdf | 97.31 kB | Adobe PDF | View/Open |
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