Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/1967
Title: Development of agrobacterium-mediated transformation method for marine microalgae (Chlorella sp. and Nannochloropsis sp.)
Authors: Willy, Yee
Keywords: QR 82 .R45 W5 2011
Willy, Yee
Tesis FST 2011
Agrobacterium
Issue Date: Feb-2011
Publisher: Terengganu : Universiti Malaysia Terengganu
Abstract: An important step in the development of an efficient Agrobacterium-medialed transformation system is the establishment of optimal conditions for efficient TDNA transfer. Parameters known to influence the efficacy of Agrobacterium tumefaciens T-DNA transfer such as pre-culture of algal cells, inoculum density, cocultivation temperature, co-cultivation duration, pH of co-cultivation medium and the concentration of acetosyringone were evaluated by monitoring transient GUS expression two days post-infection.
URI: http://hdl.handle.net/123456789/1967
Appears in Collections:Fakulti Sains dan Teknologi

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