Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/5660
Title: Genetic Diversity in Farmed Tiger Grouper Broodstock Inferred by Partial Cytochrome b Gene
Authors: Noraini Che Hassan
Abdul Hadi Abdul Aziz
Nabilah Mohamad Ali
Nur Asma Ariffin
Ivan Koh Chong Chu
Sufian Mustafa
Shahreza Md. Sheriff
Keywords: Broodstock
Epinephelus fuscoguttatus
artial Cyt-b
Tiger grouper
Issue Date: 2015
Publisher: International Journal of Chemical, Environmental & Biological Sciences (IJCEBS)
Abstract: A study was conducted to determine the genetic diversity of a farmed tiger grouper Epinephelus fuscoguttatus broodstock population located in Tanjung Demong, Terengganu using Cytochrome b (Cyt-b) gene. A total of 51 broodstock were collected and subjected to DNA extraction. PCR amplification of partial Cyt-b was conducted using a universal Cyt-b primer. The fragments were then purified, sequenced and the genetic relationship among individuals was analyzed. A total of 402 bp length of partial Cyt-b gene was obtained from each sample. Genetic distance among individuals ranged between 0.002 – 0.018. Seven haplotypes were found within the population with 2 haplotypes (Hap01 and Hap02) are commonly shared by most individuals. Phylogenetic analysis showed broodstocks is divided into two major groups. However, 47 individuals were clustered in 1 group with shallow genetic divergence. These findings indicate the tiger grouper broodstock have low genetic diversity and most of the individuals might be originating from a single population. Genetic diversity of mtDNA cyt-b of E. fuscoguttatus would be useful in tiger grouper broodstock management in breeding program for seed production.
URI: http://hdl.handle.net/123456789/5660
Appears in Collections:Journal Articles

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