Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/5843
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dc.contributor.authorLaith A.A-
dc.contributor.authorMazlan A.G-
dc.contributor.authorEffendy A.W-
dc.contributor.authorAmbak M.A-
dc.contributor.authorNadirah M-
dc.contributor.authorMuhammad T.S-
dc.contributor.authorZain S.M-
dc.contributor.authorJabar A-
dc.contributor.authorNajiah M-
dc.date.accessioned2017-04-13T08:28:48Z-
dc.date.available2017-04-13T08:28:48Z-
dc.date.issued2016-02-16-
dc.identifier.urihttp://hdl.handle.net/123456789/5843-
dc.description.abstractThis study investigated the phytochemical, antimicrobial and antioxidant properties of methanolic leaf fractionated extracts of Excoecaria agallocha. Antibacterial activity test of fractionated extract of Excoecaria agallocha was conducted via Disk diffusion assay. Micro titer broth dilution technique was performed to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Qualitative and quantitative DPPH radical scavenging activity determines the antioxidant activity in E. agallocha. Chromatographic separation was completed using thin layer chromatography (TLC) and column chromatographic techniques. Phytochemical screening of fractionated extract revealed active constituents including alkaloids, saponins, flavonoids, terpenoid and tannins. In vitro antibacterial activity of columnfractionated extract demonstrated inhibitory effect against Flavobacterium indicum, Chryseobacterium meningosepticum, Chryseobacterium indologenes and Chryseobacterium gleum isolated from diseased fish. MIC and MBC of E. agallocha fractionated extract was 1.56 mg/ml and 3.12 mg/ml, respectively. TLC-based qualitative DPPH analysis revealed antioxidant activity in E. agallocha extract, specified by yellowish stain on maroon color TLC plate. These bands indicate the presence of various compounds that could act as potential antimicrobial agents. The present study indicates that E. agallocha leaves contain active phytochemicals which inhibit bacteria and could be useful in the treatment of microbial infections in fishen_US
dc.language.isoenen_US
dc.publisherBiosciences Biotechnology Research Asiaen_US
dc.subjectExcoecaria agallochaen_US
dc.subjectAntibacterialen_US
dc.subjectAntioxidantsen_US
dc.subjectBacteriaen_US
dc.subjectTLC profilingen_US
dc.titlePhytochemical composition and in vitro antimicrobial, antioxidant activites of methanolic leaf extracts from Excoecaria Agallochaen_US
dc.typeArticleen_US
Appears in Collections:Journal Articles



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