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http://umt-ir.umt.edu.my:8080/handle/123456789/9982| Title: | Agrobacterium-mediation trannsformation of rice with omega-3- desaturase gene |
| Authors: | Muhammad Haziq Syahir Khaizuran |
| Keywords: | LP 3 FST 1 2013 Muhammad Haziq Syahir Khaizuran |
| Issue Date: | 2013 |
| Publisher: | Universiti Malaysia Terengganu |
| Abstract: | For more than two decades, acetosyringone is the phenolic compound uses in Agrobacterium-mediated transfmmation using callus de1ived from seed as a starting material. In the present study, the efficiency of vanillin in Agrobacterium-mediated transformation of omega-3-desa!urase gene into rice calli was investigated. The results showed that vanillin concentration at 200 pLM produced the highest percentage of putative transformant on the 8J1tibiotic screening medium and P-glucuroniclase (GUS) assay compared to control ancl400 ~-tM ofvanillin. The ability of vanillin in activating vir gene provides an alternali ve phenolic compound for Agrobacterium-mediated gene transformation of rice. |
| URI: | http://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/9982 |
| Appears in Collections: | Fakulti Sains dan Teknologi |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| LP 3 FST 1 2013 Abstract.pdf | 1.38 MB | Adobe PDF | View/Open | |
| LP 3 FST 1 2013 Full Text.pdf Restricted Access | 9.65 MB | Adobe PDF | View/Open Request a copy |
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