Please use this identifier to cite or link to this item: http://umt-ir.umt.edu.my:8080/handle/123456789/9982
Title: Agrobacterium-mediation trannsformation of rice with omega-3- desaturase gene
Authors: Muhammad Haziq Syahir Khaizuran
Keywords: LP 3 FST 1 2013
Muhammad Haziq Syahir Khaizuran
Issue Date: 2013
Publisher: Universiti Malaysia Terengganu
Abstract: For more than two decades, acetosyringone is the phenolic compound uses in Agrobacterium-mediated transfmmation using callus de1ived from seed as a starting material. In the present study, the efficiency of vanillin in Agrobacterium-mediated transformation of omega-3-desa!urase gene into rice calli was investigated. The results showed that vanillin concentration at 200 pLM produced the highest percentage of putative transformant on the 8J1tibiotic screening medium and P-glucuroniclase (GUS) assay compared to control ancl400 ~-tM ofvanillin. The ability of vanillin in activating vir gene provides an alternali ve phenolic compound for Agrobacterium-mediated gene transformation of rice.
URI: http://umt-ir.umt.edu.my:8080/xmlui/handle/123456789/9982
Appears in Collections:Fakulti Sains dan Teknologi

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