Establishment of anther vculture of Inddia cultivars MR 84 and MR 219 mutant-4

Abstract

A study was conducted mainly to determine the suitable media of calli induction for indica rice cultivars MR 219 Mutant-4 and MR 84. The inductions of callus using different combination of 2,4-D, NAA and kinetin were observed within 60 days. SK I media was used for anther inoculation treated with different concentration of 2,4-D, NAA and kinetin to induce callus. There was no emergence of callus for all treatment after 60 days. So, data was recorded with regard to the ability of anther to survive. For rice cultivars MR 219 Mutant-4, treatment 12 with 2.0mg/L 2,4-D, 1.0mg/L kinetin and 3.0mg/L NAA was the best treatment where I 00% of anther planted survived. On the other hand, for cultivars MR 84, treatment 2 with 0.5mg/L 2,4-D, 0.1 mg/L kinetin and 3.0mg/L NAA and treatment 11 with 2.0mg/L 2,4-D, 1.0mg/L kinetin and 3.0mg/L NAA showed highest percentage of anther survival (99.71 %). The media used in this study were not suitable for callus induction cultivars MR 84 and MR 219 Mutant-4. There was no new media establish for anther culture of rice cultivars MR 84 and MR 219 Mutant-4.