Abstract:
Anther culture in indica rice is found hardly to induce callus. The objectives of this
study are to establish anther media for calli induction of rice cultivars MR232 and
MR219 mutant 9. The seeds obtained from Rice Gene Bank, MARDI Research
Station, Seberang Perai, Pulau Pinang. Boots containing immature spikelets were used
for microspore explants. The anthers were cultured in sterilised condition. Anthers
were cultured on SKI media added with combination of 2, 4-D, kinetin and NAA at
varying concentration. The higher number of anthers survived in Treatment 6 for
MR232 at top part. Treatment 4 which containing combination of 0.75mg/L 2, 4-D,
0.5mg/L kinetin and 2mg/L NAA able to induce high calli frequency about 3.33%
derived from anther in cultivar MR232. There are high frequency of calli found in
cultivar MR232 and found to be suitable in combination of different concentration of
2, 4-D, kinetin and NAA which are combination of 0.75mg/L 2, 4-D, 0.5mg/L kinetin
and 2mg/L NAA.
