Optimization of collagen extraction from silver catfish (Pangasius sp.) waste and its properties

Abstract

The objective of this study is to optimize the collagen extraction from silver catfish (Pangasius sp.) waste using Response Surface Methodology. The effects of acetic acid concentration (M), pepsin concentration(%) and extraction time (h) were studied in order to obtain optimum collagen yield. A three- level factor face-centered central composite design (CCD) was adapted in this study resulted in twenty runs of experiments were employed. The generated model gave a quadratic fit with experimental data The optimal condition for collagen extraction from silver catfish waste were found to be at 0.2 M of acetic acid, 0.5% of pepsin and 24.7 h of extraction time. The predicted collagen yield under optimal conditions was 5.64%, while the actual experimental yield was 4.61 %. Silver catfish collagen showed the highest solubility at pH 9 and minimum solubility at pH 2. The solubility of collagen decreased gradually from 0% NaCl until 12% NaCl. The viscosity of silver catfish collagen decreased gradually at increased temperature.