Abstract:
Ammonium (Nf-4 +) toxicity is an issue of global ecological and economic importance.
Under acidic environment, high NI-Li+ concentration can cause toxicity and stress to
plants that inhibited the growth. To prevent NI-Li+ toxicity, the plants respond to a
strongly increased the antioxidant in reducing reactive oxygen species level. The
objective of this study is to determine the effect of ammonium on non-enzymatic
antioxidant, phenolics and chlorophyll content in A. simplex culture. In this study, the
effect of various NI-Li+ concentrations on A. simplex culture was investigated for 28
days after cultured in B5 medium with 0, 9.1, 18.2, 27.3, 36.5 or 45.6 mM of NI-Li+
concentration. Plant growth was measured at 7 day interval for four weeks where as
the non-enzymatic activities, chlorophyll content, total phenolic content, flavonoid
content and flavones were measured at day 1, 3, 7, 14, 21 and 28. The ascorbic acid,
carotenoid and flavonoid content were reduced with the increasing of ammonium
concentration. The a-tocopherol, chlorophyll, total phenolic content and flavones were
increased when NH/ concentration increased. This plant seems to be tolerated in
various NH4
+ concentrations.
