The effect of different concentration of sodium hydroxide in deacetylation on the physicochemical properties of chitosan extracted from mantis shrimp (Harpiosquilla harpax)

Abstract

Chitosan was prepared from mantis shrimp carapace species of harpiosquilla harpax by deacetylation process performed in 40% and 50% NaOH and both samples were maintained at 90 °C and for 5 hours. Chitosan obtained were analyzed for physicochemical properties to observe the effects of deacetylation using different concentration of sodium hydroxide (NaOH). Chitosan extracted from deacetylation by 50% NaOH (C50) showed higher degree of deacetylation than that of chitosan extracted from deacetylation by 40% NaOH (C40) which is 72.91 % while the other one is 70.60%. The yield of chitosan extracted (CSO and C40) is (51.46% and 45.0%) due more to the source of chitin and not to the effectiveness of degree of deacetylation. The moisture content for C40 and C50 is 12.607% and 14.24%, for ash content analysis,the C40 showed 0.3316% while the ash content obtained from C50 is 0.3179%. The other physicochemical analysis are; water binding capacity (C40754.0% and CS0-718.7%), fat

binding capacity (C40-344.29%) and (CS0-381.39%), apparent viscosity; (C40-1403.3 cP) and (CS0-2023.3 cP). The colour of chitosan extracted is in yellowish-white, small crisp and glitter flakes form. The different concentration of NaOH affects the physicochemical properties of chitosan extracted by affecting the degree of deacetylation.