Abstract:
The effect of media strength on flavonoid content in S. asiatica cultures was investigated. The explants were cultured in double strength, full strength and half strength of MS and B5 media separately. The flavonoids were fractionated according to the ratio of chloroform/methanol by 100% chloroform, 80:1, 40:1, 20:1 and 10:1. Thin Layer Chromatography test obtained showed that presence of flavonoid in S. asiatica in vitro.
This study found that culture medium for highest accumulation of flavonoids in S. asiatica is MS medium (full strength). Half strength MS medium shows highest ratio flavonoid content in fraction E (0.157 mg/g). Full strength of MS medium shows most favourable flavonoid production in fraction B (0.526 mg/g) and D (0.356 mg/g) crude
weight. Double Strength of MS medium shows highest flavonoid production in fraction A (0.457 mg/g). Full strength of B5 medium contains highest flavonoid content in fraction C (0.368 mg/g). Accumulation of flavonoid in S. asiatica in vitro is affected by media
strength.
