Abstract:
Typha latifolia is monocotyledonous plant which commonly grows in the wetland. It has
the ability for alleopathy response, manufacture pesticides, treatment the polluted water
and also stronger in immunosuppressive activity. This study was conducted to establish
and determine the effect of cytokinin on proliferation of T. latifolia besides to observe the
total flavone, flavonoid and phenolic content of plant. Apical tips and rhizome of T.
latifolia were collected from Simpang Pulai, lpoh was cultured for six to nine weeks in
MS basal medium with various cytokinin (1.0-5.0 mgr1
) of 6-bezyladenine (BAP), 6-
furfurylaminopurine (Kinetin/KN), Thidiazuron (TDZ) and 6-4-hydroxy-3-methyl-but-2-
enylaminopurine (Zeatin/ZEA). 1.0 mgr1 and 2.0 mgr1 BAP was induced maximum in
leaves multiplication. Transfer of rhizome to MS medium supplemented with BAP and
lndole-3-acetic acid (IAA), individually or combination, indicated that a combination 1: 1
ratio of BAP and IAA was required in leaves multiplication. In contrast, the elongation of
leaves was found highest in medium free hormone. The presence of a single 2.0 mgr1
BAP and 3.0 mgr1 TDZ and also combination of 5.0 mgr1 BAP+3.0 mgr1 IAA were
produced the greater number of new plantlets. Root induction was lower in the presence
of cytokinin but may induce higher with presence of auxin. 1.0 mgr
1
BAP+ 3.0 mgr
1
IAA
was efficient in root development. The total flavone, flavonoid and phenolic content of T.
latifolia were measured. 3.0 mgr1 BAP gave the highest content of total phenolic
compound. The highest content of flavonoids was obtained from 3.0 mgr 1 KN and
combination between 1.0 mgr1 BAP+3.0 mgr1 IAA. However, the medium free hormone
gave the highest content of total flavone than single treatment in T. latifolia. In contrast,
combination between 1.0 mgr
1
BAP+3.0 mgr
1
IAA has the highest of total flavone
content. On the basis of culture findings, we can conclude that MS media supplemented
with different concentration of cytokinins and auxins may influence the growth and
secondary metabolites in T. latifolia.
