Abstract:
Inflammation has been implicated in initiation and progression of cardiovascular
disease risk. Expression of cell surface adhesion molecule plays an important role in
adherence of monocytes or leukocytes which lead to pathogenesis of atherosclerosis.
Stevia rebaudiana is a traditional medicinal plant in South America which also as
regained popularity as natural sweetener. Stevioside is type of diterpenoid glycoside,
a bioactive compound which has been reported to exhibit pharmacological effects in
vivo, in-vitro and human studies, in which include anti-inflammatory effect. In the
present study, pre-incubation (prevention) and incubation (treatment) of stevioside on
Lipopolysaccharide (LPS)-stimulated Human Umbilical Vein Endothelial cells
(HUVECs) were explored its anti-inflammatory effect on the expression of
inflammatory marker Vascular Adhesion Molecule-I (VCAM-1) and Intercellular
Adhesion Molecule-l(ICAM-1) further investigated.
Cell Enzyme-linked
Immunosorbent assay (ELISA) was used to assess the VCAM-1 and ICAM-1
expression. MIT cytotoxicity assay was implemented in order to determine the
cytotoxicity effect of stevioside in normal primary cells, HUVECs. The present study
demonstrated that the optimum LPS condition for expression ofVCAM-1 and ICAM1
were 1.25 µg/mL and 2.5 µg/mL, respectively at 3 hours incubation time
