Effect of magnesium on phenolic contnet in cryptocoryne elliptica cultures

Abstract

Magnesium (Mg) is a component of several primary and secondary minerals in the soil. Mg act as a central atom in the chlorophyll molecule and responsible for plant leaf trapping light. Magnesium is also essential in the synthesis of chlorophyll and photosynthesis process. In this study the effect of various Mg concentrations on phenolics compound in Cryptocoryne elliptica grown tissue culture was investigated. Two month old of in vitro plantlets were aseptically transferred into modified B5 medium with Mg at 0, 12.2, 24.3, 36.5 and 48.7mM. The plantlets fresh weight, total phenolic, flavonoids and anthocyanin content were measured at every 7 days interval for 8 weeks. C. elliptica can survive after 8 weeks of culture under_ Mg deficient. Excess amount of Mg al-so not significantly alter the fresh weight of C. elliptica plantlets and higher fresh weight was in 24.3 to 48. 7mM Mg. The total phenolic, flavonoids and anthocyanin content were increased accordingly with Mg concentration. Culture of C. elliptica could growth in broad range of Mg concentration. The present of Mg also contributed to the biosynthesis of phenolics compound in this plant.