Abstract:
Cytokinin is believed can trigger cell division in Chlorel/a sp. but for production of
fatty acid remain unknown. Thus, the influence of zeatin at various concentrations;
0.7, 1.4 and 2.8mg/L were studied for their impact on growth and fatty acid
composition in Chlorella sp. (UMT-Ml) that cultured in F/2 medium with aeration.
The culture conditions were maintained as follows; salinity at 30ppt, light illustration
24 hours, temperature at 25±1 °C, and light intensity at 150μmol photon m·2s·1
• The
growth of Chlorella sp. was measured every two days until day 42 in terms of number
of cells according to the values of optical density (OD6oo). At late of stationary phase
on day 42, the cells were harvested for total oil extraction, and gas chromatography
for determination of fatty acid composition. The cells number of Ch/ore/la sp. (UMTM
1) was highest, 2.68±0.06 (x107 cells/ml) when treated with 2.8 mg/L zeatin. The
total oil content was highest, 9.96±7.57 (w/w %), in 2.8mg/L zeatin. SFA was major
saturation fatty acid found in Ch/ore/la sp. (UMT-Ml), but was inhibited when zeatin
promoted in it. For MUFA and PUFA, zeatin at 1.4mg/L enhanced the most for
production of these fatty acids. Collectively, these data suggest that zeatin was
favorable to promote growth in Ch/ore/la sp. (UMT-Ml), but no significant different
in fatty acid composition.
