Effect of zeatin on growth and fatty acid composition clorella sp. (UMT-M1)

Abstract

Cytokinin is believed can trigger cell division in Chlorel/a sp. but for production of fatty acid remain unknown. Thus, the influence of zeatin at various concentrations; 0.7, 1.4 and 2.8mg/L were studied for their impact on growth and fatty acid composition in Chlorella sp. (UMT-Ml) that cultured in F/2 medium with aeration. The culture conditions were maintained as follows; salinity at 30ppt, light illustration 24 hours, temperature at 25±1 °C, and light intensity at 150μmol photon m·2s·1 • The growth of Chlorella sp. was measured every two days until day 42 in terms of number of cells according to the values of optical density (OD6oo). At late of stationary phase on day 42, the cells were harvested for total oil extraction, and gas chromatography for determination of fatty acid composition. The cells number of Ch/ore/la sp. (UMTM 1) was highest, 2.68±0.06 (x107 cells/ml) when treated with 2.8 mg/L zeatin. The total oil content was highest, 9.96±7.57 (w/w %), in 2.8mg/L zeatin. SFA was major saturation fatty acid found in Ch/ore/la sp. (UMT-Ml), but was inhibited when zeatin promoted in it. For MUFA and PUFA, zeatin at 1.4mg/L enhanced the most for production of these fatty acids. Collectively, these data suggest that zeatin was favorable to promote growth in Ch/ore/la sp. (UMT-Ml), but no significant different in fatty acid composition.