Effect of kinetin on growth and fatty acid composition in chlorella sp. (UMT-M1)

Abstract

The effect of the kinetin on growth and fatty acid composition of Ch/orella sp. (UMTMl ) were conducted. Three different concentrations used were 0.7, 1.4 and 2.8 mg/1 kinetin. The microalgae cultured at 25°C and 24h of l SOμmol photon m-2 s- 1 for light illustration. The growth of the Ch/orella sp. in the medium treated with kinetin was observed. The 1.4 mg/1 of kinetin recorded the highest number of cells, 1.86 ± 0.08 (x107 cells/ml), after 42 days of cultivation in aeration condition, while the control show the lowest number of cells which was 1.31 ± 0.07 (x107 cells/ml). The total oil content was increased accordingly as the concentration of kinetin increased. The total saturated fatty acid (SF As) was decreased as the concentration of kinetin increased, while both mono-unsaturated (MUFAs) and polyurisaturated fatty acid (PUFAs) were increased accordingly. The major selected fatty acid was found in Chlorella sp. treated with kinetin were C16:0, C16:1, C17:0, C18:0, C18:1, C18:2, C18:3n3 and C20:0. Thus, it was concluded that kinetin can enhanced the growth of the Chlorella sp but has no significant different between fatty acid composition. Further studies must carry out to determine how biochemical stimulatant affects the fatty acid biosynthesis.