Effect of 6-benzylaminopurine on growth and fatty acids composition in chlorella sp. (UMT-M1)

Abstract

Ch/ore/la is single-celled green algae which belong to phylum Chlorophyta. Ch/ore/la species is a potential source of a wide spectrum of nutrients, including chlorophyll, carotenoids, minerals, vitamins, and long-chain polyunsaturated fatty acids (PUFAs). External plant growth hormone is one of the alternatives that can use in order to enhance the productivity of microalgae for commercial potential. This study was conducted to determine the effect of 6-benzylaminopurine (BAP); 0.0, 0. 7, 1.4, and 2.8mg/L on growth and fatty acids composition in Chlorella sp. (UMT-Ml). The isolated Chiarella sp. (UMT-M 1) was cultured and maintained under aeration at culture condition of temperature 25± 1 °C, 24 hour light illustration and l 50μmol photon m-2s-1 light intensity in culture room for 42 days. The cells were harvested at late stationary phase by flocculation and analyzed using Gas Chromatography. The growth of Chlorella sp. (UMT-M 1) was significantly influence by the present of BAP. The cells number increase accordingly with the amount of BAP used in the medium. BAP in Chlorefla sp. (UMT-Ml) culture medium show effect on the production of fatty acids profile. The highest oil content and PUF As composition was recorded in 0.7mg/L BAP medium; oil content (22.747 ± 1.522 w/w%) and PUFAs composition (35.63 ± 6.81 %). Application of exogenous such as BAP altered the growth and fatty acid profile in Chiarella sp. (UMT-M l ).