Abstract:
Osmotic stress is the main problems in aquatic plant as this stress becomes a major plant
limiting factor in decreasing water content, inhibiting the rate of photosynthesis, and
decreasing the rate of respiration. Responses of enzymatic and non- enzymatic activities
to drought in Cryptocoryne minima plantlet were observed in this study. C. minima
plantlets were cultured into Gamborg B5 media with 20% (w/v) PEG-4000 for drought
stress treatment using seven days interval and in Gamborg BS media without PEG media
used as control. The growth and activities of ascorbate peroxidase (APX), peroxidase
(POD), and catalase (CAT) as well as a-tocopherol, ascorbic acid, antocyanin, and
carotenoid were examined. Fresh weight and dry weight are increased every week along
the treatment period however the weight of treatment plants were less than that of control
plant. Drought stress induce by PEG reduce the activity of CAT and POD than that of
control. Meanwhile, the ascorbic acid and carotenoid was increased. a-T ocopherol and
APX activity shows fluctuated pattern and antocyanin show the lower concentration when
treated with PEG. Drought stress cause physiological changes on the C. minima plantlet
