Enzymatic and non-enzymatic activity of Cryptocoryne minima cultures under drought stress

Abstract

Osmotic stress is the main problems in aquatic plant as this stress becomes a major plant limiting factor in decreasing water content, inhibiting the rate of photosynthesis, and decreasing the rate of respiration. Responses of enzymatic and non- enzymatic activities to drought in Cryptocoryne minima plantlet were observed in this study. C. minima plantlets were cultured into Gamborg B5 media with 20% (w/v) PEG-4000 for drought stress treatment using seven days interval and in Gamborg BS media without PEG media used as control. The growth and activities of ascorbate peroxidase (APX), peroxidase (POD), and catalase (CAT) as well as a-tocopherol, ascorbic acid, antocyanin, and carotenoid were examined. Fresh weight and dry weight are increased every week along the treatment period however the weight of treatment plants were less than that of control plant. Drought stress induce by PEG reduce the activity of CAT and POD than that of control. Meanwhile, the ascorbic acid and carotenoid was increased. a-T ocopherol and APX activity shows fluctuated pattern and antocyanin show the lower concentration when treated with PEG. Drought stress cause physiological changes on the C. minima plantlet