The production of polisaccharide and determination of its chemical composition from marine bacteria, Acinetobacter sp. sampled at Bidong Island

Abstract

Isolation of bacteria that collected from the marine environment at Bidong Island was carried out. The pure cultured bacteria used for this study were provided by Institute Marine Biotechnology from University Malaysia Terengganu. The purpose of this study is to identify the bacterium culture on crude oil and analysis the chemical composition from polysaccharide that contain in the bacterium. From the study that has been conducted, the isolated bacteria were identified by using different type of methods. The method that had been used is gram staining, biochemical test, and REMEL identification kits. The bacterium was identified as Acinetobacter through RaplD TM NF Plus (REMEL identification kits). The bacterium, Acinetobacter yield 345.16 mg/L of crude polysaccharide from the three batch of culture medium. The analysis of chemical polysaccharide was conducted by using High Performance Liquid Chromatography (HPLC) and Paper Chromatography (PC) and its show the sugar composition obtained were glucose and mannose.