Production and study of chemical composition of polysaccharide produced from marine bacteria collected from Bidong Island

Abstract

The selected marine bacteria from Bidong Island water was used in the study of the production of polysaccharides. The selected marine bacteria used had been carried out in the investigation of identification of the species of the bacteria. This study also identified the chemical composition of the polysaccharides that produced by the selected marine bacteria. RapID NF Plus System (REMEL, USA) was used in the identification of the selected marine bacteria that then namely as Alca/igenes sp. after the test was done. The biochemical test also was done in order to identify the bacteria since the entire biochemical test characterized the characteristics of the bacteria. The average yield of the polysaccharide produced from Alca/igenes sp. was 176.0 mg per 1 L of the medium and the polysaccharides represented by white to cream in color. The determination of chemical composition of the polysaccharides was performed by using High Performance Liquid Chromatography (HPLC). From HPLC chromatogram, sugar composition that presences in crude polysaccharides of Alcaligenes sp. were rhamnose and glucose.