Production and determination of chemical composition of polysaccharide from marine bacteria (Ps.orizyhabitans) sampled in Bidong water

Abstract

Marine bacteria used in this studied were sampled in Bidong Island's water of Terengganu. It was taken from stock cultured in Laboratory of Institute of Marine Biotechnology (1MB). The bacteria were identified and were used for the production of crude polysaccharide. The product (crude polysaccharide), used to determined the sugar components present in the samples by using High Performance Liquid Chromatography (HPLC) and Paper Chromatography (PC). The selected bacteria were identified as Pseudomonas oryzihabitans by using RapID NF PLUS species identification kit. The polysaccharides yielded from the bacteria were about 265mg per 1 L culture for every batch. The analysis of sugar component in the crude polysaccharides; glucose and mannose were observed as its monomer.