Study on the masculinization of false clownfish (Amphiprion ocellaris) using 17 a-methyltestosterone

Abstract

False clownfish {Amphiprion ocellaris) is the most common marine ornamental species that is popular among hobbyist. However, the production of this species in captivity is still low. Most of the broodstocks are wild-caught and the F1 generations failed to breed in captivity. Hormone treatment has been used widely to produce monosex population in fish species. The main objective of this project is to determine if hormone can be used to masculinize this fish. Therefore, in order to achieve this objective, several studies were conducted to study the effects of: (i) immersion and oral administration of 17 a-methyltestosterone in producing male fish, (ii) pairing and non-pairing of 17 a-methyltestosterone treated fish on gonad structure of A. ocellaris.