Antibiotic and heavy metal resistance of bacteria isolated from mud crab (Scylla spp.) cultured in Setiu Wetland, Terengganu

Abstract

Seventy six of bacteria strains were successfully isolated from mud crabs (Scylla spp.) from Setiu Wetland, Terengganu. Their sensitivity of the bacteria isolates towards the selected antibiotics and testing the bacteria resistant against heavy metals has been done and determined. Twenty one types of selected antibiotics discs at different concentrations were used to determine the susceptibility bacterial isolates. Among the isolated bacteria, Aeromonas hydrophila, Hafaia alvei, Vibrio parahaemolyticus, Vibrio alginolyticus, Chromobacterium violaceum, Vibrio cholerae, Vibrio metschnikovii, Plesiomonas shigelloides, Burkholderia pseudomallei, Escherichia coli, Pseudomonas aeruginosa, Morganella morganii, Acinetobacter baumannii, Yersinia enterocolitica, Proteus vulgaris, Shewanella putrefaciens and Enterobacter sakazakii were isolated. Highly found were Aeromonas hydrophila (25 strains), Hafaia alvei (14 strains), Vibrio parahaemolyticus ( 14 strains) followed by other bacteria such as species. All successfully bacteria isolated also has been tested for their ability to tolerate heavy metal cadmium (II) chloride (CdCl2), potassium (II) chromate (K2Cr207) and cuprum sulfate (CuS04). A heavy metals resistance pattern Cd > Cu> K was observed in bacteria isolated in the study. ln the antibiotic test, bacteria showed highly susceptibility to ampicilin, amoxycillin, colistin sulphate, oleandomycin and lincomycin. Few of bacteria isolated showed highly resistance to doxycycline, erythromycin, novobiocin, spiramycin, oxytetracycline, tetracycline, furazolidone, fosfomycin, nitrofuranton, florfenicol, flumequire and chloramphenicol and I 00% bacteria isolated showed resistance to oxolinic acid, kanamycin, dan nalidixin acid.