Abstract:
Pacific Bean Donax, Donax faba is commonly found in coastal zone, especially in East
Coast of Peninsular Malaysia. It can be obtained in monsoon seasons within August to
December every year. This species is not cultured commercially, but people from
coastal inhabitants of Malaysia traditionally consume Pacific Bean Donax harvested
from wild stock. In the present study, Pacific Bean Donax was collected at Pantai
Penarik, Terengganu. The samples were collected by using benthic dredge and brought
to laboratory to be examined. During the transportation, samples were kept in the
aerated aquarium containing water from the natural habitat. At the laboratory, the
muscle and intra fluid were collected in sterile plastic bags containing Physiological
saline were added to plastic bags. The samples were homogenized vigorously prior to
ten fold serial dilution (l o-1, 10-
2
, 10-
3
, 10
-4
and I 0-5). Then, the I 00µ1 aliquots of each
dilution were spread plated onto TCBS agar (Merck, Germany) for bacterial isolation.
The plates were incubated for 24 to 48 hours at 37
°
C. The green and yellow colonies
were selected and maintained on TSA (2% NaCl) (Merck, Germany). Sixteen isolates of
Vibrio spp. were successfully isolated and identified using series of morphological,
biochemical, and physiological tests.
