Abstract:
Siakap or Lates calcarifer is one of the important cultured fish in Malaysia. Because of
the significant value, a study was done to determine the gene expression profiling of
cage cultured and pond cultured Siakap, Lates calcarifer and to identify the
differentially expressed genes between cage and pond cultured Siakap, Lates calcarifer.
Sample of fish ranged from 300 to 800 gram was obtained from two different cultured
conditions. Several steps have been taken starting from the RNA extraction, followed by
reverse transcription. Then, the cDNA obtained were subjected to PCR amplification by
using five arbitrary primers, which are arbitrary primer 3, 6, 9, 12, and 15. Results
shows differentiation in gene profile between pond and cage cultured siakap after PCR
amplification by using arbitrary primer 3, 6, and 9. The amplification by using arbitrary
primer ACP 3 produced two and four fragments for cage and pond cultured siakap
cDNA respectively. The difference can be seen at the size of 450bp, 330bp and 250bp.
On the other hand, the amplification by using arbitrary primer ACP 6 produced seven
cDNA fragments. The difference can be seen at the size of 400bp, 300bp and l 50bp.
Meanwhile, by using primer ACP 9, there were five cDNA fragments produced and the
difference can be found at the size of 400bp, 270bp and 1 OObp.
