Gene expression profiling between pond and cage cultured siakap (Lates calcarifer)

Abstract

Siakap or Lates calcarifer is one of the important cultured fish in Malaysia. Because of the significant value, a study was done to determine the gene expression profiling of cage cultured and pond cultured Siakap, Lates calcarifer and to identify the differentially expressed genes between cage and pond cultured Siakap, Lates calcarifer. Sample of fish ranged from 300 to 800 gram was obtained from two different cultured conditions. Several steps have been taken starting from the RNA extraction, followed by reverse transcription. Then, the cDNA obtained were subjected to PCR amplification by using five arbitrary primers, which are arbitrary primer 3, 6, 9, 12, and 15. Results shows differentiation in gene profile between pond and cage cultured siakap after PCR amplification by using arbitrary primer 3, 6, and 9. The amplification by using arbitrary primer ACP 3 produced two and four fragments for cage and pond cultured siakap cDNA respectively. The difference can be seen at the size of 450bp, 330bp and 250bp. On the other hand, the amplification by using arbitrary primer ACP 6 produced seven cDNA fragments. The difference can be seen at the size of 400bp, 300bp and l 50bp. Meanwhile, by using primer ACP 9, there were five cDNA fragments produced and the difference can be found at the size of 400bp, 270bp and 1 OObp.