Cloning and identification of 300 bp partial expressed gene fragment isolated from muscle tissue of african catfish, Clarias gariepinus

Abstract

A study was done to clone and identify the expressed gene from muscle tissue of African catfish, Clarias gariepinus. In thisi study, RNA was extracted from the muscle tissue of African catfish and 300 bp fragment was produced using primer 5 in amplification of DD RT-PCR. The fragment was cloned, sequenced and it was named as P5300. Sequenced analysis using BLAST programmed at the NCBI (http://www.ncbi.nlm.nih.gov/) showed that there was no significant match with the gene deposited in the GenBank indicating that P5300 fragment might be an unknown gene or specific gene for muscle tissue of the African catfish. This study shows that it is possible to identify new genes which have never been reported before. Due to this, further study should be done in order to know the gene of P5300 fragment and its important role in the muscle tissue of African catfish for a better understanding of the gene expression and regulation.