Cloning and identification of~950 basepair fragment in tiger grouper (epinephelus fuscoguttatus) in bali population

Abstract

A study to clone DNA marker of Tiger Grouper, Epinephelus fuscogu,ttatus from Bali population was carried out. In this study, DNA of E. fuscoguttatus from Bali, Kedah, Langkawi and Sabah population were taken. The DNA was extracted from the muscle tissue and the genomic DNA was then used as the template for Polymerase Chain Reaction (PCR) to amplify. The marker should be identify first from these population by using primer OPA 1, OPA 11 and OPA 20 since Rosmawati (2007) had did primer screening before. Based on PCR products, OP A 20, produced a monomorphic band which is very clearly seen from Bali and was chosen. This DNA fragment then was isolated from gel and inserted into a vector pTZ57R/T for nucleotide sequencing. A 952 hp sequence was obtained. This sequence was 99 % similar to the Enterobacteria phage T4 which is a complete genome and phage T4 pseT gene for polynucleotide kinase (pnk) with accession number AF 158101.6 and X03007. l respectively. For four descriptions obtained from BLAST had E value of 0.0. It note that E value is low. The lower the E-value, the more significant the hit.