Bacteriological study in association with antibiotic resistance and heavy metal of bacterial population from giant freshwater prawn larvae waste

Abstract

A study was carried out to determine bacterial count on larvae waste of Giant freshwater prawn (Macrobrachium rosenbergii) collected from the bottom tank at University Malaysia Terengganu Marine Hatchery, and their resistance to various antibiotics and heavy metals. The bacteria were isolated using non-selective agar Trypticase Soy Agar (TSA) and selective agar were Thiosulfate Citrate Bile Sucrose TPC (TCBS), Mac Conkey, Pseudomonas Aeromonas Selective Agar base (GSP), Eosin Methylene Blue (EMB) and Xylose Lysine Deoxycholate (XLD). The plate counts ranged from 7 x 10 2 to 1.53 x 10 4 bacterial per disc for larvae waste of M.rosenbergii. The bacteria genera found on larvae waste were Vibrio, Pseudomonas, Aeromonas and Escherichia. This bacteria were the most frequently found encountered genera based on appearance and growth of colonies on selective agar. A total of 50 strain bacterial isolates isolated from larvae waste of M.rosenbergii was examined the antibiotic resistance and results showed that the bacterial resistance to lincomycelin was highest followed by oleandomycin, novobiocin, spiramycin, deoxycyclin, ampicilin, amoxicillin and tetracycline. Most of the isolates were tolerant to all heavy metal test with the pattern of Hg = Cd = Cr > Cu. These results indicate that bacteria isolate resistance to antibiotic and heavy metal posing a potential public health risk.