Abstract:
Water samples were taken along Teluk Kalong in order to study the bacteria found in
these water. Industrial .discharge at Teluk Kalong was sampled for three sampling site.
The objectives of study are to identify bacteria population and to investigate the presence
of indicator bacteria along the waste discharge point in waters of Teluk Kalong. Three
type of bacteria used as indicator for the study which are Escherechia coli, Vibrio sp. and
Enterococcus sp. Colony Forming Unit (CFU) was used to determine the number of
bacteria presence at sampling site. The physical characteristic, morphology of bacteria
and the number of colonies was observed and recorded. E. coli shows the highest number
compared to others. All of these bacteria can affect health problem to human and cause
public health. However, the water parameter not used as identification factor for this
study. Polymerase Chain Reaction method using primer 63 F and 1389 R was used to
identify type of bacteria presence in sampling site. The electrophoresis gel was stained
with ethium bromide (EtBr2) and purified using Bioteke Purification Kit before send to
l 51 Base Sdn. Bhd. for sequencing. The identified bacteria were Vibrio parahaemolyticus
and Halomonas aquamarina. V parahaemolyticus can cause gastrointestinal illness while
presence of H. aquamarina indicate that the surrounding water may contain high
concentration of iron.
