Identification of bacteria in waters of Teluk Kalong, Kemaman, Terengganu

Abstract

Water samples were taken along Teluk Kalong in order to study the bacteria found in these water. Industrial .discharge at Teluk Kalong was sampled for three sampling site. The objectives of study are to identify bacteria population and to investigate the presence of indicator bacteria along the waste discharge point in waters of Teluk Kalong. Three type of bacteria used as indicator for the study which are Escherechia coli, Vibrio sp. and Enterococcus sp. Colony Forming Unit (CFU) was used to determine the number of bacteria presence at sampling site. The physical characteristic, morphology of bacteria and the number of colonies was observed and recorded. E. coli shows the highest number compared to others. All of these bacteria can affect health problem to human and cause public health. However, the water parameter not used as identification factor for this study. Polymerase Chain Reaction method using primer 63 F and 1389 R was used to identify type of bacteria presence in sampling site. The electrophoresis gel was stained with ethium bromide (EtBr2) and purified using Bioteke Purification Kit before send to l 51 Base Sdn. Bhd. for sequencing. The identified bacteria were Vibrio parahaemolyticus and Halomonas aquamarina. V parahaemolyticus can cause gastrointestinal illness while presence of H. aquamarina indicate that the surrounding water may contain high concentration of iron.