Abstract:
Light intensity, temperature, and culture medium are important factors regulating
the growth of microalgae. This study was conducted at Universiti Malaysia
Terengganu. Marine microalgae Tetrase/mis sp. was isolated and cultured in two
different media such as Conway and KW21 at different condition indoor and
outdoor. This study investigated the effect of different culture media and culture
condition on the growth and proximate composition of Tetraselmis sp. At indoor
culture, Tetrase/mis sp. had 24 hours continuous fluorescent lamp while outdoor
culture the growth of Tetraselmis sp. was depended on natural sunlight 12: 12
light: dark cycles. The growth of Tetraselmis sp. was determined by using two
parameters which was cell density ( cells/ml) and optical density (ABS), while for
the proximate composition was determined by using dry weight percentage (%).
The growth and proximate composition-Protein, lipid and carbohydrate of all
culture treatments were determined at stationary phase. Tetraselmis sp. was
growing slightly higher in Conway medium when compare KW2 l medium at
indoor (laboratory/controlled) culture. Then, outdoor (natural) culture condition
was shown, higher grown in KW2 l medium when compare Conway medium.
The percentage (%) of protein in Tetraselmis sp. cultured using KW2 l medium
had significantly higher compared with Conway medium for both culture conditions. Lipid accumulation in Tetrase/mis sp. was slightly higher cultured in
KW21 medium (16.66%) under indoor condition while Conway medium was
higher cultured under outdoor condition (12.8%). The carbohydrates productions
seem slightly higher in Conway medium (30 .18%) under indoor culture while
KW21 medium (27.2%) slightly higher in under outdoor condition. From the
result, can concluded that Tetraselmis sp. had no significant differences for
growth in both Conway and KW21 media under indoor and outdoor culture
condition because of influenced by some physical factor such as light intensity
and temperature as well as nutrient composition such as phosphate, nitrogen and
trace metals.
