Growth and proximate composition of tropical Tetraselmis sp. culture with different media in indoor and outdoor culture

Abstract

Light intensity, temperature, and culture medium are important factors regulating the growth of microalgae. This study was conducted at Universiti Malaysia Terengganu. Marine microalgae Tetrase/mis sp. was isolated and cultured in two different media such as Conway and KW21 at different condition indoor and outdoor. This study investigated the effect of different culture media and culture condition on the growth and proximate composition of Tetraselmis sp. At indoor culture, Tetrase/mis sp. had 24 hours continuous fluorescent lamp while outdoor culture the growth of Tetraselmis sp. was depended on natural sunlight 12: 12 light: dark cycles. The growth of Tetraselmis sp. was determined by using two parameters which was cell density ( cells/ml) and optical density (ABS), while for the proximate composition was determined by using dry weight percentage (%). The growth and proximate composition-Protein, lipid and carbohydrate of all culture treatments were determined at stationary phase. Tetraselmis sp. was growing slightly higher in Conway medium when compare KW2 l medium at indoor (laboratory/controlled) culture. Then, outdoor (natural) culture condition was shown, higher grown in KW2 l medium when compare Conway medium. The percentage (%) of protein in Tetraselmis sp. cultured using KW2 l medium had significantly higher compared with Conway medium for both culture conditions. Lipid accumulation in Tetrase/mis sp. was slightly higher cultured in KW21 medium (16.66%) under indoor condition while Conway medium was higher cultured under outdoor condition (12.8%). The carbohydrates productions seem slightly higher in Conway medium (30 .18%) under indoor culture while KW21 medium (27.2%) slightly higher in under outdoor condition. From the result, can concluded that Tetraselmis sp. had no significant differences for growth in both Conway and KW21 media under indoor and outdoor culture condition because of influenced by some physical factor such as light intensity and temperature as well as nutrient composition such as phosphate, nitrogen and trace metals.