Wound healing activity of the methanolic extract of Melaleuca cajuputi leaves on Lates calcarifer

Abstract

This study was conducted to determine the 96h LC5o of Melaleuca cajuputi extract on Lates calcarifer and the value was found to be 102.06mg/L. The methanolic extract of M cajuputi was used to treat 3 2 wounded fish for 24 hours to investigate the wound healing activity. The result of this study showed that the methanolic extract of M cajuputi leaves can promote wound healing activity in wounded skin of L. calcarifer at the concentration of 20mg/L. The methanolic extract showed remarkable wounds healing activity by fasten the proliferation and re-epithelialisation phase by facilitating the proliferation of epithelial cell or by increasing the viability of the epithelial cell. At the concentration of 40mg/L and 60mg/L, the methanolic extract of M. cajuputi resulted delayed in wound healing activity. This probably caused by the presence of free radicals oxygen of higher concentration that can lead to obvious tissue damage.