Abstract:
Gram negative strains, A 22 l o-
6 l and A 22 l o-
6 2 were isolated from
mangrove sediment collected at Setiu. Both strains are potential
polyhydroxyalkanoate (PHA) producer after shown positive results in screening for
PHA production with Nile red stain when emitted pink fluorescence under UV light.
Biosynthesis of PHA by both strains were carried out with glucose, fructose and oleic
acid as carbon sources. GC analysis on dried sample of both strains from biosynthesis
enriched with glucose and fructose detected production of medium chain length PHA.
Strain A22 10-6 1 detected in production of C io, poly(3-hydroxydecanoate) P(3HD),
C 12, poly(3-hydroxydodecanoate) P(3HD0) and C 14 , poly(3-hydroxytetradecanoate)
P(3HTD) while A 22 1 o-6 2 detected for production of P(3HD) and P(3HTD).
