Abstract:
The Asian green arowana (Scleropages formosus) is an important ornamental fish.
Currently, sex identification of this species is still a major problem in arowana farming
due to lack sexual dimorphism which hinders sex selection during culture. In this study,
the protein pattern of the blood plasma which consists of major hormones of the arowana
were determined with SOS-PAGE, revealing an apparent band at approximately 200kDa
in arowana samples with unknown gender. Immunoprobing with a monoclonal Anti-Vtg
(Zebrafish) failed to detect the protein of interest, perhaps due to weak cross-reactivity
and specificity of the antibody against arowana. This band possibly represents
Vitellogenin (Vtg) based on the molecular weight and reference from other piscine
species.
