Abstract:
Treated with eyestalk ablation and continuous feeding of squids which is Loligo spp,
female mud crab, Scylla olivacea were induced for ovarian maturation till Stage 4 of
ovary maturation stage in order to perform In-vitro trials. After the female crabs were
fully mature enough Stage 4, the crabs were selected and been dissected. There are eight
trials that have been done and the trials were divided into two parts which are by using
sperms in spermatheca of female crabs and the other one is by using sperms in vas
deferens of male crabs. Stages 4 ovary was combined with sperms and were maintained
in small aquarium with 30
°
C of water temperature set by using water heater. Daily
observation was done by using compound and advance microscope while the fertilization
rate and early stage of embryonic development were calculated and being identified. The
fertilization rates were different in each trial. Trial 1, 2 and 3 were failed and starting
from Trial 4 until Trial 8, the fertilization rate starting to give favorable result.
