Identification of tenualosa SP. in Perak Riever using morphometric measurement, meristic count and coi gene analysis

Abstract

Tenualosa tali and Tenualosa macrura can be found in Sarawak water and have higher commercial value in Malaysia (Blaber et al., 1996; Phillip, 2001). According to Perikanan (2012), Tenualosa sp. also can be found in Perak River, but the species of this genus still 1midentified. All samples were collected using net in December 2013 and February 2014 and the species was identified based on distinguished character of morphological and meristic count. DNA was extracted from five sample of Tenualosa sp. PCR amplification of 600 bp partial fragment of CO 1 gene was amplified using CO 1 marker. This PCR fragment was then sequenced and analysed using Mega version 5. The result from BLAST showed that there are the sequences have high similarity with CO 1 gene. The phylogenetic analysis was constructed using Neighbour Joining tree and Maximum Likelihood tree.