Abstract:
Actinomycetes are known to produces chemically varied compounds with a broad range
of biological acti ity. In this project, nine actinomycetes that have been isolated from
Bidong island marine sediment but two potential actinomycetes as anticancer were
identified as Streptomyces sp. and Nocardia sp which are A6 and A9 respectively. Potato
Dextrose Agar (PDA) and Casein Starch Agar (CSA) were used as culture and isolation
media. From cytotoxic test, they showed IC50 value while the rest does not showed IC50
due to their reading were below 50 percent of cytotoxic index. That showed that
actinomycetes strains number A 1, A2, A3, A4, AS, A 7 and A8 have low inhibition
activity against MCF-7 cell line. They showed value greater than 100 µg/mL. Their
sample concentration have to be increased a lot to reach IC50• A6 showed the highest
cytotoxic followed by A9. A6 showed IC50 value (1.5 mg/mL) followed by A9 (5.3
mg/mL) at 24 hour test. For identification, actinomycetes strain no A6 and A9 (which are
showed active anticancer activity) were identified through morphological characteristics
due unobtainable PCR product. They were moderately active to inhibit cancer.
