Anticancer activity of actinomycetes isolated from Bidong Island, Terennganu against MCF-7 cell line

Abstract

Actinomycetes are known to produces chemically varied compounds with a broad range of biological acti ity. In this project, nine actinomycetes that have been isolated from Bidong island marine sediment but two potential actinomycetes as anticancer were identified as Streptomyces sp. and Nocardia sp which are A6 and A9 respectively. Potato Dextrose Agar (PDA) and Casein Starch Agar (CSA) were used as culture and isolation media. From cytotoxic test, they showed IC50 value while the rest does not showed IC50 due to their reading were below 50 percent of cytotoxic index. That showed that actinomycetes strains number A 1, A2, A3, A4, AS, A 7 and A8 have low inhibition activity against MCF-7 cell line. They showed value greater than 100 µg/mL. Their sample concentration have to be increased a lot to reach IC50• A6 showed the highest cytotoxic followed by A9. A6 showed IC50 value (1.5 mg/mL) followed by A9 (5.3 mg/mL) at 24 hour test. For identification, actinomycetes strain no A6 and A9 (which are showed active anticancer activity) were identified through morphological characteristics due unobtainable PCR product. They were moderately active to inhibit cancer.