Abstract:
Diseases such as the Taura syndrome and White Spot Syndrom Disease (�SSV)
causes mass mortalities of cultured shrimp worldwide,resulting to severe economic
losses. The white leg shrimp Litopenaeus vannamei (pacific white leg shrimp) a
popular cultured species due their rapid growth, high survival and tolerance to wide
range of environmental (Briggs et al., 2004). Nonetheless L.
vannamei is affected by
Vibrios, the gram-negative bacteria that cause Vibriosis in shrimp and other
crustacean. V. anguillarum and V.harveyi represent two main Vibrio spp found to
infect penaeid shrimp. In this study, Thiosulfate citrate Bile Salt Sucrose agar (TCBS)
was used to isolate Vibrio spp which occur in L.
vannamei cultured in the East coast of
Peninsula Malaysia, work that includes verification of other bacteria that grow on
TCBS agar. Six different bacteria species were found on TCBS agar, with their
molecular sequence revealing Bacillus amyloliquefaciens, B.thuringiensis, B.subtilis,
Shewanella haliotis, Vibrio parahaemolyticus and Pseudomonas aeruginosa.. This
observation strongly indicates that bacteria other than Vibrios grow on TSBC agar.
