Identification of bacteria isolated on tcb agar from the white leg shrimp litopenaeus cultured in the east coast of Penisular Malaysia

Abstract

Diseases such as the Taura syndrome and White Spot Syndrom Disease (�SSV) causes mass mortalities of cultured shrimp worldwide,resulting to severe economic losses. The white leg shrimp Litopenaeus vannamei (pacific white leg shrimp) a popular cultured species due their rapid growth, high survival and tolerance to wide range of environmental (Briggs et al., 2004). Nonetheless L. vannamei is affected by Vibrios, the gram-negative bacteria that cause Vibriosis in shrimp and other crustacean. V. anguillarum and V.harveyi represent two main Vibrio spp found to infect penaeid shrimp. In this study, Thiosulfate citrate Bile Salt Sucrose agar (TCBS) was used to isolate Vibrio spp which occur in L. vannamei cultured in the East coast of Peninsula Malaysia, work that includes verification of other bacteria that grow on TCBS agar. Six different bacteria species were found on TCBS agar, with their molecular sequence revealing Bacillus amyloliquefaciens, B.thuringiensis, B.subtilis, Shewanella haliotis, Vibrio parahaemolyticus and Pseudomonas aeruginosa.. This observation strongly indicates that bacteria other than Vibrios grow on TSBC agar.