Genetic variation of Malaysia gold arowana (scleropages formosus) using PCR-RELP on cytochrome b gene

Abstract

A study of genetic variation of Malaysian Gold Arowana or known as Scleropages formosus using PCR-RFLP method on cytochrome b gene. The project was done by using the fish scales which were taken from Bukit Merah, Perak. The aim of this study is to determine the genetic variation of Scleropages formosus using different types of restriction enzymes those are Hae III, Mbo I, Nde I, Alu I and Msp I. DNA was extracted and was amplified because for RFLP method large quantity of DNA is needed, the DNA was amplified by using Cytochrome b primer. After the amplification of DNA was done, purification of the PCR sample was done to remove the impurities which might affect the result of the study. Final procedure was the RFLP method by using Restriction enzymes, Hae III, Mbo I, Nde I, Alu I and Msp l the DNA base pairs in PCR was about 1100 bp and this was continued with RFLP method.