Abstract:
Oxidative stress refers to situations that occur when there is an imbalance of
production and neutralization of reactive oxygen species (ROS) by antioxidants
caused by excessive generation of ROS and/or loss of antioxidant that can cause
oxidative damage and glutathione reductase (GR) is used as biomarker that acts as
protector in the mechanism against that damage. Bivalves are the bio-indicator in this
study because they are widely used in environmental toxicology studies that capable
to show a rapid antioxidant response towards pollutant and as a human food sources.
This study focus on oysters (Crassostrea iredalei) and mussels (Anadara ova/is)
which are the common species that mostly found in Setiu Wetland, Terengganu. The
samples were analysing on tissues-specific relation of oxidative stress to GR by
measuring the reduction of NADPH at 340nm. Before that, protein mass
determination using Bradford method is needed to estimate the specific activity of the
GR. The outcome shows that the GR activities in gills for both species are greater
than digestive glands.
