Detection of aquatic pollutants using luminescent bactteria

Abstract

Nowadays, luminescent bacteria as a sensing organism to detect the aquatic pollutants have been widely used. In this study, luminescent bacteria were identified and the effect of different concentrations of pollutants towards the density of luminescent bacteria was carried out. The luminescent bacteria were isolated from the ink of the squids. Then, the Gram staining was done to observe the shape of the luminescent bacteria. The bacteria were identified using VITEK. Besides that, the bacteria were tested with different NaCl concentration (0, 1, 2, 3, 4 and 5 % (w/v)) and at temperature (4, 26, 28, 37 and 40 ° C). Then, toxicity test was carried out with 1.5 ml of toxicants Cd, Zn Cb, HgS04, Pb(N03)2, diazinon and malathion (2, 4, 6, 8 and 10 ppm) were added to 1.5 ml of bacterial suspension in a cuvette. As for control, 1.5 ml of saline solution was added to 1.5 ml of bacterial suspensions.