Characterisation And Relative Expression Of A Gene Encoding Vwd-Kazal In Different Developmental Stages Of Oocyte Maturation In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man, 1879)

Abstract

Breeding of giant freshwater prawn, Macrobrachium rosenbergii is dependent on the availability of matured female broodstocks. The insufficient supply of matured female broodstocks has become a major constraint for sustainable production of larvae. To date, the induction of ovarian maturations of M. rosenbergii is based on eyestalk ablation technique which may cause high mortality rate and lower seed ratio of the broodstock. Molecular approaches based on ovarian maturation related genes, vWD domain and three Kazal-type domains gene (vWD-Kazal) that can be potentially used for the induction of ovarian maturations in broodstock were investigate. This research aims to achieve the following objectives: 1)