Preparation of Eel (Monopterus Albus) Protein Hydrolysate Using Alcalase® And Its Physicochemical, Antioxidant And Anticancer Properties

Abstract

The study aims to determine the optimum condition of eel protein hydrolysate (EPH) produced using Alcalase® and its physicochemical, antioxidant and anticancer properties. The hydrolysis condition was optimized using Response Surface Methodology (RSM) by applying four factors (time, temperature, pH, Alcalase® concentration), 3-levels Central Composite Design (CCD) with six central points. The chemical and amino acid compositions, structural properties, solubility, emulsifying and foaming properties, water holding and fat binding capacity of EPH were determined. The EPH was separated through ultrafiltration membranes (10 kDa, 5 kDa and 3 kDa). The ferric thiocyanate, thiobarbituric reactive substance, reducing power, ferrous ion chelating activity and 1,2-diphenylpicrylhydrazyl radical scavenging activity of fractionated EPH were also determined. The anticancer activity was determined using MCF-7 cell lines. In order to obtain the optimum yield, degree of hydrolysis (DH) and DPPH activity, the optimum hydrolysis condition suggested was 84.02 min, 50.18°C, pH 7.89 and 2.26% Alcalase® concentration